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Sample GSM1477461 Query DataSets for GSM1477461
Status Public on Jan 07, 2015
Title REP_R1881_rep2
Sample type RNA
 
Source name immortalized primary fetal human epididymis cells
Organism Homo sapiens
Characteristics cell type: SV-40 transformed primary human fetal epididymis cells
Treatment protocol Cells are cultured in phenol-free CMRL-1066 medium containing 10% dextran coated-charcoal (C6241, Sigma) - stripped FBS for 72h prior to stimulation with 1 nM R1881 (NLP005005MG, PerkinElmer, Waltham, MA) or vehicle for a further 16h.
Growth protocol Cells are cultured in Primaria flasks (Falcon) in CMRL 1066 medium, containing 20% foetal calf serum (FCS) (Gibco, UK); L-glutamine (4mM); insulin (0.2 unit/ml); cholera toxin (10-10M) and hydrocortisone (1μg/ml), all from Sigma. Cultures were routinely maintained at 37°C in a humidified 5% CO2 incubator.
Extracted molecule total RNA
Extraction protocol TRIzol® (Life Technologies) used for RNA extraction.
Label Cy3
Label protocol RNA samples are sent to Mogene company for further processing and data generation.
 
Hybridization protocol RNA samples are sent to Mogene company for further processing and data generation.
Scan protocol RNA samples are sent to Mogene company for further processing and data generation.
Data processing RNA samples are sent to Mogene company for further processing and data generation.
 
Submission date Aug 13, 2014
Last update date Jan 07, 2015
Contact name Ann Harris
E-mail(s) ann-harris@northwestern.edu
Organization name Northwestern University
Street address 2430 N Halsted
City Chicago
State/province Illinois
ZIP/Postal code 60614
Country USA
 
Platform ID GPL17077
Series (2)
GSE59783 Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function
GSE60381 Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function [Agilent expression]

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
3xSLv1 2.36526
A_19_P00315452 10.06886
A_19_P00315459 7.81334
A_19_P00315482 3.81222
A_19_P00315492 4.48236
A_19_P00315493 6.15407
A_19_P00315502 9.67513
A_19_P00315506 8.62922
A_19_P00315518 2.65398
A_19_P00315519 2.33637
A_19_P00315524 5.54677
A_19_P00315528 7.05787
A_19_P00315529 6.34567
A_19_P00315538 2.42402
A_19_P00315541 2.44124
A_19_P00315543 5.65991
A_19_P00315550 10.35110
A_19_P00315551 9.40219
A_19_P00315554 4.28480
A_19_P00315581 13.08054

Total number of rows: 50728

Table truncated, full table size 1076 Kbytes.




Supplementary file Size Download File type/resource
GSM1477461_US83800207_253949412187_S01_GE1_1105_Oct12_2_1.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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