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Sample GSM1467707 Query DataSets for GSM1467707
Status Public on Aug 14, 2014
Title Suz12_PS7_1
Sample type SRA
 
Source name Suz12_PS7
Organism Drosophila melanogaster
Characteristics tissue: embryo
age: 5-13h
tissue subtype: parasegment 7
chip antibody: anti-SU(Z)12
chip antibody ref.: Müller et al 2002
Growth protocol Eggs were harvested 5-13h after laying, nuclei extracted and marked parasegment-specific nuclei sorted by FACS
Extracted molecule genomic DNA
Extraction protocol Nuclei were sonicated and ChIP performed using the appropriate antibody.
Libraries were prepared as described in Bowman et al., 2013.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina MiSeq
 
Data processing OLB 1.9.0 / RTA 1.13 used for base-calling
CHIPseq reads aligned to dm3 using BWA, allowing up to 1 mismatch and otherwise default settings
Data were filtered for reads aligning to multiple positions, pcr duplicates, and inferred fragment size <100 or >800.
Genome_build: dm3
Supplementary_files_format_and_content: Wig files generated using SPP's "smoothed.density" function, following default guidelines estimating peak separation distance and removing low quality and anomalous tags.
 
Submission date Aug 07, 2014
Last update date May 15, 2019
Contact name Ruslan Sadreyev
Organization name Massachusetts General Hospital
Department Molecular Biology
Lab Bioinfomatics
Street address 185 Cambridge Street
City Boston
State/province Massachusetts
ZIP/Postal code 02114
Country USA
 
Platform ID GPL16479
Series (1)
GSE55257 H3K27 modifications define segmental regulatory domains in the Drosophila bithorax complex.
Relations
BioSample SAMN02954552
SRA SRX671950

Supplementary file Size Download File type/resource
GSM1467707_Suz12_PS7_1.bw 29.0 Mb (ftp)(http) BW
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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