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Sample GSM1466234 Query DataSets for GSM1466234
Status Public on Dec 26, 2014
Title hPGC male week 7 rep2
Sample type SRA
Source name hPGC isolated from developmental week 7 genital ridge
Organism Homo sapiens
Characteristics cell type: hPGC isolated from developmental week 7 genital ridge
facs: TNAP and cKIT+
gender: Male
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with the PicoPure RNA Isolation Kit. Total RNA (0.5-2 ng) was reverse transcribed and amplified into cDNA using Ovation RNA-Seq System V2 (Nugen) according to manufacturer’s instructions.
Amplified cDNA was sonicated into 250 bp by Covaris S220 Focused-ultrasonicators. Subsequently, RNASeq library was generated with 500 ng of fragmented cDNA using Ovation Ultralow DR Multiplex System (Nugen). Library was quantified by KAPA Library Quantification Kit (Kapa Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description Primary cells
Data processing Low quality bases (q < 20) from the 3' end and adapter sequences were removed with the TrimGalore wrapper scripts (
Reads were mapped to the human (GRCh37/hg19) reference genome with Tophat 2.0.12 (
Genome_build: GRCh37/hg19
Supplementary_files_format_and_content: Processed data files contain read counts per RefSeq transcripts generated by using the HTSeq 0.6.1 python package (
Submission date Aug 06, 2014
Last update date Oct 20, 2020
Contact name Sabine Dietmann
Organization name Washington University School of Medicine
Street address 4565 McKinley Avenue
City St Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platform ID GPL11154
Series (1)
GSE60138 SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Reanalyzed by GSE95736
Reanalyzed by GSM4838075
BioSample SAMN02953222
SRA SRX670402

Supplementary file Size Download File type/resource
GSM1466234_hPGC.E48_M.txt.gz 116.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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