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Sample GSM1464558 Query DataSets for GSM1464558
Status Public on Mar 12, 2015
Title CB_WT_d3a_2
Sample type RNA
 
Source name cerebellum
Organism Mus musculus
Characteristics age: 10-11 weeks of age
brain region: cerebellum
genotype: Dnmt3a flx/flx
Treatment protocol NA
Growth protocol Mice were housed under standard conditions in the animal facility
Extracted molecule total RNA
Extraction protocol Cerebellum was dissected from 8-10 week old MeCP2 R306C or wild-type littermate controls. RNA was trizol and extracted using Qiagen Rneasy purification with on column DNase steps.
Label biotin
Label protocol Fragmentation and labeling were carried out using the The AmbionĀ® WT Expression Kit
 
Hybridization protocol Hybridization was carried out as described in the GeneChipĀ® Whole Transcript (WT) Sense Target Labeling Assay Manual v4 Chapter 5
Scan protocol Arrays were scanned with the Affymetrix GeneChip Scanner 3000
Data processing Data were processed using Genespring software (Agilent). Gene level expression were summarized use RMA16.
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date Aug 04, 2014
Last update date Mar 12, 2015
Contact name Harrison Wren Gabel
E-mail(s) gabelh@pcg.wustl.edu
Organization name Harvard Medical School
Department Neurbiology
Lab Michael Greenberg
Street address 220 longwood avenue
City brookline
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6096
Series (2)
GSE60070 Length-dependent gene misregulation in Rett syndrome (Dnmt3a)
GSE60077 Length-dependent gene misregulation in Rett syndrome

Data table header descriptions
ID_REF
VALUE RMA16 signal estimates from Genespring v12.6

Data table
ID_REF VALUE
6747308 9.427778
6747309 10.481743
6747314 9.866476
6747326 6.2107434
6747343 9.827854
6747354 10.9331045
6747364 10.235823
6747471 8.261225
6747472 8.981214
6747478 8.117227
6747497 8.328247
6747504 6.161508
6747515 9.016053
6747577 7.3244123
6747641 9.086128
6747696 4.9640083
6747747 8.239114
6747786 8.730627
6747805 9.151091
6747837 8.666071

Total number of rows: 17831

Table truncated, full table size 302 Kbytes.




Supplementary file Size Download File type/resource
GSM1464558_CB_WT_d3a_2.CEL.gz 23.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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