|
Status |
Public on Mar 12, 2015 |
Title |
CB_WT_d3a_2 |
Sample type |
RNA |
|
|
Source name |
cerebellum
|
Organism |
Mus musculus |
Characteristics |
age: 10-11 weeks of age brain region: cerebellum genotype: Dnmt3a flx/flx
|
Treatment protocol |
NA
|
Growth protocol |
Mice were housed under standard conditions in the animal facility
|
Extracted molecule |
total RNA |
Extraction protocol |
Cerebellum was dissected from 8-10 week old MeCP2 R306C or wild-type littermate controls. RNA was trizol and extracted using Qiagen Rneasy purification with on column DNase steps.
|
Label |
biotin
|
Label protocol |
Fragmentation and labeling were carried out using the The AmbionĀ® WT Expression Kit
|
|
|
Hybridization protocol |
Hybridization was carried out as described in the GeneChipĀ® Whole Transcript (WT) Sense Target Labeling Assay Manual v4 Chapter 5
|
Scan protocol |
Arrays were scanned with the Affymetrix GeneChip Scanner 3000
|
Data processing |
Data were processed using Genespring software (Agilent). Gene level expression were summarized use RMA16. probe group file: MoEx-1_0-st-v1.r2.pgf meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
|
|
|
Submission date |
Aug 04, 2014 |
Last update date |
Mar 12, 2015 |
Contact name |
Harrison Wren Gabel |
E-mail(s) |
gabelh@pcg.wustl.edu
|
Organization name |
Harvard Medical School
|
Department |
Neurbiology
|
Lab |
Michael Greenberg
|
Street address |
220 longwood avenue
|
City |
brookline |
State/province |
Massachusetts |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL6096 |
Series (2) |
GSE60070 |
Length-dependent gene misregulation in Rett syndrome (Dnmt3a) |
GSE60077 |
Length-dependent gene misregulation in Rett syndrome |
|