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Sample GSM14510 Query DataSets for GSM14510
Status Public on Oct 08, 2004
Title FRD440 2
Sample type RNA
Source name Calu-3 human lung epithelial cells
Organism Homo sapiens
Extracted molecule total RNA
Description Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 10**8 CFUs per ml of one of the bacterial strains (FRD1, FRD440, FRD875, or FRD1234) for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy (Qiagen) for microarray hybridization. Control samples of Calu-3 cells were treated similarly, but not infected with bacteria. Single array image analysis was performed using MAS 5 (Affymetrix) to derive probeset intensities and detection p-values. All arrays were globally scaled to 500.
Keywords = CF, Cystic Fibrosis, Pseudomonas, Alginate, Flagellin
Submission date Dec 19, 2003
Last update date Oct 28, 2005
Contact name Josyf Mychaleckyj
Phone 336-713-7540
Organization name Wake Forest Univ School of Medicine
Department Center for Human Genomics
Street address
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
Platform ID GPL96
Series (1)
GSE923 Pseudomonas aeruginosa infection of Calu-3 human lung epithelial cells

Data table header descriptions
ID_REF Probe set identifier on U133A array
VALUE MAS 5 derived signal, a measure of the abundance of a transcript
ABS_CALL MAS 5 detection call, default parameters. Probeset was present (P), absent (A), marginal (M), or no call (NC). Present call threshold is 0.05.
DETECTION P-VALUE MAS 5 detection p-value, the significance level of the detection call

Data table
1007_s_at 4126.2 P 0.000266
1053_at 477.6 P 0.000562
117_at 59.1 A 0.302547
121_at 1592.8 P 0.000959
1255_g_at 40.2 A 0.47938
1294_at 190.3 A 0.066865
1316_at 118.1 P 0.013092
1320_at 24.6 A 0.581931
1405_i_at 11.8 A 0.724538
1431_at 47.4 A 0.175989
1438_at 76.1 A 0.250724
1487_at 588.8 P 0.031336
1494_f_at 251.2 A 0.089405
1598_g_at 353.2 A 0.189687
160020_at 387.9 P 0.035163
1729_at 435.5 P 0.000266
177_at 190.3 P 0.008689
1773_at 89.7 A 0.438361
179_at 700 P 0.021866
1861_at 217.9 P 0.014937

Total number of rows: 22283

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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