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Sample GSM14506 Query DataSets for GSM14506
Status Public on Oct 08, 2004
Title FRD1234 1
Sample type RNA
Source name Calu-3 human lung epithelial cells
Organism Homo sapiens
Extracted molecule total RNA
Description Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 10**8 CFUs per ml of one of the bacterial strains (FRD1, FRD440, FRD875, or FRD1234) for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy (Qiagen) for microarray hybridization. Control samples of Calu-3 cells were treated similarly, but not infected with bacteria. Single array image analysis was performed using MAS 5 (Affymetrix) to derive probeset intensities and detection p-values. All arrays were globally scaled to 500.
Keywords = CF, Cystic Fibrosis, Pseudomonas, Alginate, Flagellin
Submission date Dec 19, 2003
Last update date Oct 28, 2005
Contact name Josyf Mychaleckyj
Phone 336-713-7540
Organization name Wake Forest Univ School of Medicine
Department Center for Human Genomics
Street address
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
Platform ID GPL96
Series (1)
GSE923 Pseudomonas aeruginosa infection of Calu-3 human lung epithelial cells

Data table header descriptions
ID_REF Probe set identifier on U133A array
VALUE MAS 5 derived signal, a measure of the abundance of a transcript
ABS_CALL MAS 5 detection call, default parameters. Probeset was present (P), absent (A), marginal (M), or no call (NC). Present call threshold is 0.05.
DETECTION P-VALUE MAS 5 detection p-value, the significance level of the detection call

Data table
1007_s_at 3455.6 P 0.000388
1053_at 383.5 P 0.007543
117_at 117.2 A 0.098054
121_at 1685 P 0.002617
1255_g_at 9.3 A 0.621816
1294_at 395.9 M 0.060419
1316_at 139.8 P 0.011447
1320_at 102.2 A 0.47938
1405_i_at 9.1 A 0.979337
1431_at 81.7 A 0.066865
1438_at 137 A 0.438361
1487_at 824.4 P 0.043968
1494_f_at 286.9 A 0.11716
1598_g_at 567 A 0.204022
160020_at 476.4 P 0.035163
1729_at 339.5 P 0.000388
177_at 201.1 P 0.024711
1773_at 118.7 A 0.339558
179_at 895.5 A 0.07383
1861_at 195.4 A 0.127645

Total number of rows: 22283

Table truncated, full table size 594 Kbytes.

Supplementary data files not provided

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