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Sample GSM14505 Query DataSets for GSM14505
Status Public on Oct 08, 2004
Title FRD1 4
Sample type RNA
Source name Calu-3 human lung epithelial cells
Organism Homo sapiens
Extracted molecule total RNA
Description Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 10**8 CFUs per ml of one of the bacterial strains (FRD1, FRD440, FRD875, or FRD1234) for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy (Qiagen) for microarray hybridization. Control samples of Calu-3 cells were treated similarly, but not infected with bacteria. Single array image analysis was performed using MAS 5 (Affymetrix) to derive probeset intensities and detection p-values. All arrays were globally scaled to 500.
Keywords = CF, Cystic Fibrosis, Pseudomonas, Alginate, Flagellin
Submission date Dec 19, 2003
Last update date Oct 28, 2005
Contact name Josyf Mychaleckyj
Phone 336-713-7540
Organization name Wake Forest Univ School of Medicine
Department Center for Human Genomics
Street address
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
Platform ID GPL96
Series (1)
GSE923 Pseudomonas aeruginosa infection of Calu-3 human lung epithelial cells

Data table header descriptions
ID_REF Probe set identifier on U133A array
VALUE MAS 5 derived signal, a measure of the abundance of a transcript
ABS_CALL MAS 5 detection call, default parameters. Probeset was present (P), absent (A), marginal (M), or no call (NC). Present call threshold is 0.05.
DETECTION P-VALUE MAS 5 detection p-value, the significance level of the detection call

Data table
1007_s_at 3136.7 P 0.000266
1053_at 361.8 P 0.00418
117_at 93.8 A 0.162935
121_at 1444.5 P 0.001141
1255_g_at 77 A 0.107301
1294_at 276.5 A 0.07383
1316_at 185.9 P 0.011447
1320_at 30.8 A 0.52062
1405_i_at 34.6 A 0.388782
1431_at 71.7 A 0.11716
1438_at 103.8 A 0.189687
1487_at 511.5 P 0.035163
1494_f_at 309.7 P 0.019304
1598_g_at 524.3 A 0.218983
160020_at 544.3 P 0.039365
1729_at 235.5 P 0.005643
177_at 206 P 0.006532
1773_at 170.6 A 0.32083
179_at 891.9 P 0.019304
1861_at 179.3 P 0.02786

Total number of rows: 22283

Table truncated, full table size 593 Kbytes.

Supplementary data files not provided

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