|
| Status |
Public on Nov 30, 2014 |
| Title |
TU_BRCA1_3 [aCGH] |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
human DNA reference pool
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: self-made DNA pool, from blood samples of ten healthy female donors
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted by Proteinase K treatment with subsequent phenol-chloroform extraction
|
| Label |
Cy5-dUTP
|
| Label protocol |
Standard Agilent Genomic DNA labeling kit Plus Protocol without enzymatic restriction
|
| |
|
| Channel 2 |
| Source name |
PrimaryTumorTissue_BRCA1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Tumor
sample type: fresh-frozen
subtype: BRCA1-mutated tumor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted by Proteinase K treatment with subsequent phenol-chloroform extraction
|
| Label |
Cy3-dUTP
|
| Label protocol |
Standard Agilent Genomic DNA labeling kit Plus Protocol without enzymatic restriction
|
| |
|
| |
| Hybridization protocol |
Standard Agilent Protocol
|
| Scan protocol |
Arrays were scanned using Agilent microarray scanner according to the manufacturer´s instructions
|
| Data processing |
For image analysis, default CGH settings of the Feature Extraction software (Agilent) were applied. Output files of the Feature Extraction were further investigated by Agilent´s CHG data analysis software, Genomic-Workbench.
algorithm ADM2
|
| |
|
| Submission date |
Jul 30, 2014 |
| Last update date |
Nov 30, 2014 |
| Contact name |
Jessica Rößler |
| Organization name |
Medical School Hannover
|
| Department |
Pathology
|
| Street address |
Carl-Neuberg Str. 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10123 |
| Series (2) |
| GSE59902 |
DNA methylation study in breast cancer [aCGH] |
| GSE59903 |
DNA methylation study in breast cancer |
|
