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Sample GSM14499 Query DataSets for GSM14499
Status Public on Oct 08, 2004
Title CONTROL 2
Sample type RNA
 
Source name Calu-3 human lung epithelial cells
Organism Homo sapiens
Extracted molecule total RNA
 
Description Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 10**8 CFUs per ml of one of the bacterial strains (FRD1, FRD440, FRD875, or FRD1234) for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy (Qiagen) for microarray hybridization. Control samples of Calu-3 cells were treated similarly, but not infected with bacteria. Single array image analysis was performed using MAS 5 (Affymetrix) to derive probeset intensities and detection p-values. All arrays were globally scaled to 500.
Keywords = CF, Cystic Fibrosis, Pseudomonas, Alginate, Flagellin
 
Submission date Dec 19, 2003
Last update date Oct 28, 2005
Contact name Josyf Mychaleckyj
E-mail(s) jmychale@wfubmc.edu
Phone 336-713-7540
Organization name Wake Forest Univ School of Medicine
Department Center for Human Genomics
Lab MYCHALECKYJ
Street address
City Winston-Salem
State/province NC
ZIP/Postal code 27157
Country USA
 
Platform ID GPL96
Series (1)
GSE923 Pseudomonas aeruginosa infection of Calu-3 human lung epithelial cells

Data table header descriptions
ID_REF Probe set identifier on U133A array
VALUE MAS 5 derived signal, a measure of the abundance of a transcript
ABS_CALL MAS 5 detection call, default parameters. Probeset was present (P), absent (A), marginal (M), or no call (NC). Present call threshold is 0.05.
DETECTION P-VALUE MAS 5 detection p-value, the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1007_s_at 3811 P 0.000219
1053_at 500.3 P 0.001354
117_at 34.3 A 0.458816
121_at 1281.1 P 0.000959
1255_g_at 15.9 A 0.438361
1294_at 164.8 P 0.048995
1316_at 250.7 P 0.003585
1320_at 13.4 A 0.697453
1405_i_at 5.6 A 0.937979
1431_at 74.5 A 0.081337
1438_at 84.7 A 0.35869
1487_at 473.3 P 0.043968
1494_f_at 237.5 P 0.035163
1598_g_at 475.9 A 0.081337
160020_at 375.7 P 0.021866
1729_at 388.7 P 0.000219
177_at 288.2 P 0.003067
1773_at 49.8 A 0.697453
179_at 450.6 P 0.017001
1861_at 209.2 M 0.060419

Total number of rows: 22283

Table truncated, full table size 592 Kbytes.




Supplementary data files not provided

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