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Sample GSM1442861 Query DataSets for GSM1442861
Status Public on Nov 25, 2016
Title XPZAM_replicate3
Sample type RNA
Source name Choriodecidua, zone overlying the cervix before 32 weeks of gestation
Organism Homo sapiens
Characteristics tissue: choriodecidua
zone: overlying the cervix
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tissues, firstly crushed in Trizol (Life Technologies, Carlsbad, CA), using a Qiagen RNeasy Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug of Total RNA using the Low Input Quick Amp Labeling Kit according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol 0.6 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 25 µl containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 25 µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Sureprint G3 Human GE 8*60K (G4851A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using GE1_107_Sep09 scanning protocol (Scan resolution 2um)
Description Gene expression in zone of altered morphology
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028004_D_F_20110325) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Jul 23, 2014
Last update date Nov 26, 2016
Contact name Céline Méhats
Organization name Inserm
Department U1016
Street address 24 rue du Faubourg St jacques
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL13607
Series (1)
GSE59686 Gene profiling of the zone of altered morphology of choriodecidua overlying the cervix before labor in preterm samples

Data table header descriptions
VALUE Normalized signal intensity

Data table
4 6.499096173
5 6.297429574
6 5.406148041
7 9.835384072
8 10.74172229
9 4.959671611
10 5.307640612
11 11.08441178
12 8.462412223
13 8.374400431
14 9.263569641
15 7.116634891
16 4.841721979
17 5.75229524
18 4.959671611
19 8.168436368
20 7.630106793
21 7.85364006
24 6.228876068
25 8.245923352

Total number of rows: 58717

Table truncated, full table size 1016 Kbytes.

Supplementary file Size Download File type/resource
GSM1442861_SG11370001_252800421172_20140521095758_S001_GE1_1105_Oct12_2_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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