|
Status |
Public on Aug 08, 2014 |
Title |
H3K27Ac_MPP.ucsc |
Sample type |
SRA |
|
|
Source name |
Multipotent Progenitor (MPP)
|
Organism |
Mus musculus |
Characteristics |
cell type: Multipotent Progenitor (MPP) passages: In vivo FACS purified cells strain: C57BL/6 chip antibody: H3K27Ac
|
Growth protocol |
Cells were fixated and FACS sorted according to stablished markers
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 1500 |
|
|
Data processing |
Basecalls performed using CASAVA version 1.4 Genome_build: mm9 Supplementary_files_format_and_content: wig files were generated using …; Scores represent …
|
|
|
Submission date |
Jul 21, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Ido Amit |
E-mail(s) |
ido.amit@weizmann.ac.il
|
Phone |
972-8-9343338
|
Organization name |
Weizmann Institute of Science
|
Department |
Immunology
|
Street address |
234 Herzl st.
|
City |
Rehovot |
ZIP/Postal code |
760001 |
Country |
Israel |
|
|
Platform ID |
GPL18480 |
Series (2) |
GSE59636 |
Chromatin state dynamics during blood formation (ChIP-Seq) |
GSE60103 |
Chromatin state dynamics during blood formation |
|
Relations |
BioSample |
SAMN02929020 |
SRA |
SRX658376 |