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Sample GSM1440999 Query DataSets for GSM1440999
Status Public on Jan 08, 2020
Title 101039_B_7
Sample type RNA
 
Source name B
Organism Homo sapiens
Characteristics blood draw date: 7
age group: Old
phenotype: NR
immport_expsamp_acc: ImmPort:ES494659
Extracted molecule total RNA
Extraction protocol Total RNA from PBMC, T Cell, B Cell samples was extracted using the RNeasy mini kit (Qiagen, CA)
Label biotin
Label protocol Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols. Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol. The BeadChips were scanned on the Illumina Iscan.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 5997321086_J
Data processing The data were normalised using quantile normalisation with lumi in R
 
Submission date Jul 21, 2014
Last update date Jun 03, 2020
Contact name Hailong Meng
Organization name Yale University
Street address 300 George Str
City New Haven
State/province CT
ZIP/Postal code 06525
Country USA
 
Platform ID GPL10558
Series (1)
GSE65440 Gene expression analysis of Influenza vaccine response in Young and Old - Year 1 - B and T cells

Data table header descriptions
ID_REF
VALUE quantile and then log2 normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 6.433768796 0.8272727
ILMN_2055271 6.747202262 0.002597403
ILMN_1736007 6.548649237 0.2649351
ILMN_2383229 6.491133926 0.5727273
ILMN_1806310 6.554138882 0.238961
ILMN_1779670 6.439596377 0.8051948
ILMN_1653355 6.525192995 0.3948052
ILMN_1717783 6.414062748 0.8844156
ILMN_1705025 6.487742162 0.5935065
ILMN_1814316 6.612826122 0.07792208
ILMN_2359168 6.568875409 0.1883117
ILMN_1731507 6.391763443 0.9428571
ILMN_1787689 6.529551271 0.3727273
ILMN_3241953 7.146233667 0
ILMN_1745607 6.338881232 0.9857143
ILMN_2136495 6.465300666 0.7116883
ILMN_1668111 6.547437448 0.2714286
ILMN_2295559 6.504852489 0.4896104
ILMN_1735045 6.456633715 0.7467533
ILMN_1680754 6.602052287 0.1

Total number of rows: 47231

Table truncated, full table size 1511 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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