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| Status |
Public on Jul 31, 2015 |
| Title |
Whole brain of neonatal male mice treated with vehicle control |
| Sample type |
RNA |
| |
|
| Source name |
neonatal male mice brains, vehicle control injection
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
tissue: Whole brain
treatment: vehicle control injection
strain: C57BL/6
|
| Treatment protocol |
We prepared neonatal mice (C57BL/6J) injected intraperitoneally with TP (1mg in 20uL sesame oil per mice) and vehicle control (sesame oil only 20uL per mice) at postnatal 2 day (at the period of mice brain sex differentiation) and obtained mRNA from these mice whole brain at postnatal 6day.
|
| Growth protocol |
Healthy grown neonatal C57BL/6 mice were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the GeneChip 3'IVT Express Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized to the microarrays according to the manufacturer’s protocol with Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanning was preformed as described elsewhere (Irizarry et al, Nucleic Acids Res 31, e15, 2003), using Affymetrix GeneArray Scanner 3000 7G with GCOS software.
|
| Description |
Gene expression data from pooled RNA obtained from whole brains obtained from six neonatal male mice treated with vehicle control by intraperitoneal injection.
|
| Data processing |
The intensity data was background-subtracted and normalized with the robust multi-array analysis (RMA; Irizarry et al. Nucleic Acids Res 31(4): e15, 2003) via R/Bioconductor affy 1.28.1 package using default analysis settings.
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| |
|
| Submission date |
Jul 14, 2014 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Yutaka Nakachi |
| E-mail(s) |
nakachiy@kumamoto-u.ac.jp
|
| Organization name |
Kumamoto University
|
| Department |
Faculty of Life Sciences
|
| Lab |
Department of Molecular Brain Science
|
| Street address |
1-1-1 Honjo, Chuo-ku
|
| City |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE59375 |
Gene expression profile of the neonatal female mouse brain after administration of testosterone propionate. |
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