|
| Status |
Public on Jul 15, 2014 |
| Title |
#007 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
T-ALL clone #007 from thymus
|
| Organism |
Mus musculus |
| Characteristics |
cell type: T-cell acute lymphoblastic leukemia
|
| Treatment protocol |
no treatment
|
| Growth protocol |
T-ALL clone developed in mice transplanted with TRK-transduced LSK cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted accoding Qiagen GmbH, Hilden, Germany
|
| Label |
Cye3-dUTP
|
| Label protocol |
According to manufacture's instructions (Agilent) and purifion by Amicon Ultra 30k filters (Millipore, Billerica, MA, USA)
|
| |
|
| Channel 2 |
| Source name |
reference DNA (spleen)
|
| Organism |
Mus musculus |
| Characteristics |
tissue type: spleen
|
| Treatment protocol |
no treatment
|
| Growth protocol |
T-ALL clone developed in mice transplanted with TRK-transduced LSK cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted accoding Qiagen GmbH, Hilden, Germany
|
| Label |
Cye5-dUTP
|
| Label protocol |
According to manufacture's instructions (Agilent) and purifion by Amicon Ultra 30k filters (Millipore, Billerica, MA, USA)
|
| |
|
| |
| Hybridization protocol |
According to manufacturer's instructions (Agilent), 4x180k
|
| Scan protocol |
Microarray slides were scanned immediately after washing using an Agilent microarray scanner G2505B at a resolution of 2 µm
|
| Data processing |
Feature extraction and Genomic Workbench (Agilent), algorithm ADM2 .
|
| |
|
| Submission date |
Jul 14, 2014 |
| Last update date |
Jul 15, 2014 |
| Contact name |
Doris Johanna Steinemann |
| E-mail(s) |
steinemann.doris@mh-hannover.de
|
| Phone |
+495115324669
|
| Organization name |
Hannover Medical School
|
| Department |
Institute of Human Genetics
|
| Lab |
Array-Lab
|
| Street address |
Carl-Neuberg-Str.1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10449 |
| Series (1) |
| GSE59367 |
Hyperactivation of mTORC1 and mTORC2 by multiple oncogenic events causes addiction to eIF4E-dependent mRNA translation in T-cell leukemia |
|
