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Sample GSM1419272 Query DataSets for GSM1419272
Status Public on Feb 09, 2017
Title FUCO1
Sample type RNA
Source name human gastric cancer in the presence of fucoidan
Organism Homo sapiens
Characteristics cell line: MKN45
cell type: gastric cancer cells
cell line source: stomach, liver metastasis, epithelial-like adenocarcinoma in a 62 year-old Japanese female
treated with: fucoidan
Treatment protocol Cells were cultured in DMEM with 10% FBS. For fucoidan treatment, culture medium was replaced with fresh medium mixed with fucoidan (1 mg/ml), and cells were cultured until the assay.
Growth protocol Cell growth assay was performed by counting cell numbers using a hemocytometer.
Extracted molecule total RNA
Extraction protocol Total RNA was collected from cultured MKN45 cells using the RNeasy mini kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
Hybridization protocol Agilent one-color gene expression hyb/wash protocol
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Jun 24, 2014
Last update date Apr 23, 2018
Contact name Miwa Yoshimoto
Organization name Tottori University
Street address 36-1 Nishi-cho
City Yonago
ZIP/Postal code 683-8504
Country Japan
Platform ID GPL16699
Series (1)
GSE58774 Anti-proliferation activity of fucoidan on MKN45 gastric cancer cells and downregulation of phosphorylated ASK1, a cell-cycle-regulated cyclin-dependent kinase
Reanalyzed by GSE113533

Data table header descriptions
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
1 1.104546e+005
2 3.432297e+000
3 3.348731e+000
4 4.120284e+003
5 2.919346e+001
6 4.434911e+000
7 1.459508e+001
8 2.166494e+001
9 1.153895e+005
10 3.513681e+002
11 4.605591e+002
12 6.990644e+003
13 1.486760e+002
14 3.395307e+000
15 1.090406e+001
16 3.391813e+000
17 2.498548e+002
18 1.398907e+002
19 2.717151e+003
20 4.865691e+003

Total number of rows: 62976

Table truncated, full table size 1219 Kbytes.

Supplementary file Size Download File type/resource
GSM1419272_3-FUCO1.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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