NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1416929 Query DataSets for GSM1416929
Status Public on Jan 20, 2015
Title 5234_CD4_Memory_48 [Affymetrix]
Sample type RNA
 
Source name Human Memory CD4 T Cell
Organism Homo sapiens
Characteristics activation: anti-CD3/CD28 beads, 48 hours
Treatment protocol Purified naïve and memory CD4 T cells were cultured in RPMI supplemented with 10% FBS, 100 U/ml penicillin and 100mg/ml streptomycin at 37C and 5% CO2 with anti-CD3/CD28 activator beads (Invitrogen) for 0 or 48 hours.
Growth protocol Naïve and memory CD4 cells were negatively selected using magnetic beads (Stem Cell Technologies).
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cell pellets using an All Prep kit (QIAGEN).
Label biotin
Label protocol Biotinylated cDNA were prepared using the Ambion WT Expression Kit (Ambion Life Technologies) following manufacturer's instructions. cDNA was fragmented and labeled with GeneChip WT Terminial Labeling kit (Affymetrix).
 
Hybridization protocol Following labelling, 2.5 ug of cDNA were hybridized for 17 h at 48C on HuGene 1.1ST peg arraya (Affymetrix)
Scan protocol Washing and staining and scanning done per the Gene Titan MC protocol for HuGene 1.1ST peg arrays.
Description Gene expression data for anti-CD3/CD28 activated human memory CD4 T cells
Data processing The data were analyzed with Partek Genomics Suite (Partek Incorporated) and was Robust Multi-array Average (RMA) normalized
 
Submission date Jun 19, 2014
Last update date Jan 23, 2015
Contact name Daniel Salomon
E-mail(s) dsalomon@scripps.edu
Organization name The Scripps Research Institute
Department Molecular & Experimental Medicine
Lab Salomon
Street address 10550 N Torrey Pines Rd, MEM-241
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL11532
Series (2)
GSE58684 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation [Affymetrix]
GSE59860 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7892501 7.37038
7892502 5.18326
7892503 4.02003
7892504 7.61464
7892505 2.98081
7892506 6.27566
7892507 5.29475
7892508 7.48096
7892509 11.6675
7892510 5.35138
7892511 4.27454
7892512 4.81833
7892513 4.82915
7892514 11.2757
7892515 8.85537
7892516 4.13376
7892517 6.32751
7892518 2.4147
7892519 5.7782
7892520 8.4222

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1416929_083011_KK_HuGene1.1ST_5234_CD4_Memory_48hr_H05.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap