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Sample GSM1416918 Query DataSets for GSM1416918
Status Public on Jan 20, 2015
Title 5211_CD4_Memory_0 [Affymetrix]
Sample type RNA
 
Source name Human Memory CD4 T Cell
Organism Homo sapiens
Characteristics activation: None
Treatment protocol Purified naïve and memory CD4 T cells were cultured in RPMI supplemented with 10% FBS, 100 U/ml penicillin and 100mg/ml streptomycin at 37C and 5% CO2 with anti-CD3/CD28 activator beads (Invitrogen) for 0 or 48 hours.
Growth protocol Naïve and memory CD4 cells were negatively selected using magnetic beads (Stem Cell Technologies).
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cell pellets using an All Prep kit (QIAGEN).
Label biotin
Label protocol Biotinylated cDNA were prepared using the Ambion WT Expression Kit (Ambion Life Technologies) following manufacturer's instructions. cDNA was fragmented and labeled with GeneChip WT Terminial Labeling kit (Affymetrix).
 
Hybridization protocol Following labelling, 2.5 ug of cDNA were hybridized for 17 h at 48C on HuGene 1.1ST peg arraya (Affymetrix)
Scan protocol Washing and staining and scanning done per the Gene Titan MC protocol for HuGene 1.1ST peg arrays.
Description Gene expression data for unactivated human naïve CD4 T cells
Data processing The data were analyzed with Partek Genomics Suite (Partek Incorporated) and was Robust Multi-array Average (RMA) normalized
 
Submission date Jun 19, 2014
Last update date Jan 23, 2015
Contact name Daniel Salomon
E-mail(s) dsalomon@scripps.edu
Organization name The Scripps Research Institute
Department Molecular & Experimental Medicine
Lab Salomon
Street address 10550 N Torrey Pines Rd, MEM-241
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL11532
Series (2)
GSE58684 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation [Affymetrix]
GSE59860 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7892501 6.92765
7892502 6.25199
7892503 5.1713
7892504 6.81227
7892505 3.67183
7892506 8.50721
7892507 4.81835
7892508 7.67286
7892509 11.052
7892510 6.56842
7892511 4.27946
7892512 4.82748
7892513 5.4705
7892514 10.3989
7892515 8.0282
7892516 3.67524
7892517 7.23104
7892518 2.96579
7892519 5.38204
7892520 8.64197

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1416918_091311_KK_HuGene1_1ST_5211_CD4_Memory_0hr_B07.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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