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Sample GSM1416914 Query DataSets for GSM1416914
Status Public on Jan 20, 2015
Title 4922_CD4_Memory_0 [Affymetrix]
Sample type RNA
 
Source name Human Memory CD4 T Cell
Organism Homo sapiens
Characteristics activation: None
Treatment protocol Purified naïve and memory CD4 T cells were cultured in RPMI supplemented with 10% FBS, 100 U/ml penicillin and 100mg/ml streptomycin at 37C and 5% CO2 with anti-CD3/CD28 activator beads (Invitrogen) for 0 or 48 hours.
Growth protocol Naïve and memory CD4 cells were negatively selected using magnetic beads (Stem Cell Technologies).
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cell pellets using an All Prep kit (QIAGEN).
Label biotin
Label protocol Biotinylated cDNA were prepared using the Ambion WT Expression Kit (Ambion Life Technologies) following manufacturer's instructions. cDNA was fragmented and labeled with GeneChip WT Terminial Labeling kit (Affymetrix).
 
Hybridization protocol Following labelling, 2.5 ug of cDNA were hybridized for 17 h at 48C on HuGene 1.1ST peg arraya (Affymetrix)
Scan protocol Washing and staining and scanning done per the Gene Titan MC protocol for HuGene 1.1ST peg arrays.
Description Gene expression data for unactivated human naïve CD4 T cells
Data processing The data were analyzed with Partek Genomics Suite (Partek Incorporated) and was Robust Multi-array Average (RMA) normalized
 
Submission date Jun 19, 2014
Last update date Jan 23, 2015
Contact name Daniel Salomon
E-mail(s) dsalomon@scripps.edu
Organization name The Scripps Research Institute
Department Molecular & Experimental Medicine
Lab Salomon
Street address 10550 N Torrey Pines Rd, MEM-241
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL11532
Series (2)
GSE58684 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation [Affymetrix]
GSE59860 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7892501 7.05901
7892502 5.97012
7892503 4.13229
7892504 6.99505
7892505 3.34714
7892506 8.50992
7892507 4.52627
7892508 7.5803
7892509 11.2375
7892510 6.62313
7892511 4.20167
7892512 5.80348
7892513 6.19012
7892514 10.4967
7892515 7.84041
7892516 4.11357
7892517 6.87094
7892518 4.68986
7892519 5.91327
7892520 8.89426

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1416914_083011_KK_HuGene1.1ST_4922_CD4_Memory_0_hr_B05.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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