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Sample GSM1416908 Query DataSets for GSM1416908
Status Public on Jan 20, 2015
Title 4922_CD4_Naive_0 [Affymetrix]
Sample type RNA
 
Source name Human Naive CD4 T Cell
Organism Homo sapiens
Characteristics activation: None
Treatment protocol Purified naïve and memory CD4 T cells were cultured in RPMI supplemented with 10% FBS, 100 U/ml penicillin and 100mg/ml streptomycin at 37C and 5% CO2 with anti-CD3/CD28 activator beads (Invitrogen) for 0 or 48 hours.
Growth protocol Naïve and memory CD4 cells were negatively selected using magnetic beads (Stem Cell Technologies).
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cell pellets using an All Prep kit (QIAGEN).
Label biotin
Label protocol Biotinylated cDNA were prepared using the Ambion WT Expression Kit (Ambion Life Technologies) following manufacturer's instructions. cDNA was fragmented and labeled with GeneChip WT Terminial Labeling kit (Affymetrix).
 
Hybridization protocol Following labelling, 2.5 ug of cDNA were hybridized for 17 h at 48C on HuGene 1.1ST peg arraya (Affymetrix)
Scan protocol Washing and staining and scanning done per the Gene Titan MC protocol for HuGene 1.1ST peg arrays.
Description Gene expression data for unactivated human naïve CD4 T cells
Data processing The data were analyzed with Partek Genomics Suite (Partek Incorporated) and was Robust Multi-array Average (RMA) normalized
 
Submission date Jun 19, 2014
Last update date Jan 23, 2015
Contact name Daniel Salomon
E-mail(s) dsalomon@scripps.edu
Organization name The Scripps Research Institute
Department Molecular & Experimental Medicine
Lab Salomon
Street address 10550 N Torrey Pines Rd, MEM-241
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL11532
Series (2)
GSE58684 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation [Affymetrix]
GSE59860 Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7892501 6.85727
7892502 6.68094
7892503 4.08134
7892504 6.94848
7892505 3.12236
7892506 9.21807
7892507 4.00744
7892508 7.16074
7892509 11.1368
7892510 5.80675
7892511 3.66932
7892512 7.54308
7892513 5.86228
7892514 10.0572
7892515 8.59414
7892516 3.69825
7892517 6.82534
7892518 4.42216
7892519 6.48273
7892520 9.12168

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1416908_083011_KK_HuGene1.1ST_4922_CD4_Naive_0hr_A05.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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