|
| Status |
Public on May 06, 2015 |
| Title |
X8942326001_PO2.3 |
| Sample type |
genomic |
| |
|
| Source name |
Post-obese_isolated_cells-2_years
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell type: Fat cell
disease state: postobese
age: 42
bmi: 27.8
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was prepared from fat cells using the QiAamp DNA Mini kit (cat no. 51304, Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina protocol for Human Methylation 450K DNA Analysis bead chip
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation 450K Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
4966c
Unmethylated and methylated signal intensities provided in signal_intensities.txt Series supplementary file
|
| Data processing |
data was normalized and analyzed using Genom Studio software, R packages and Qlucore software.
|
| |
|
| Submission date |
Jun 18, 2014 |
| Last update date |
May 06, 2015 |
| Contact name |
Ingrid Dahlman |
| E-mail(s) |
indranil.sinha.2@ki.se
|
| Organization name |
Karolinska Institute
|
| Department |
Department of Medicine
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| ZIP/Postal code |
14157 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE58622 |
The fat cell epigenetic signature in post-obese women is characterized by global hypomethylation and differential DNA methylation of adipogenesis genes |
|
