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Sample GSM1414423 Query DataSets for GSM1414423
Status Public on Mar 01, 2015
Title IC462T
Sample type genomic
 
Source name Serous ovarian tumor
Organism Homo sapiens
Characteristics tissue: Tumor epithelium
tumour histology: SEROUS
diagnosis: LOW GRADE CARCINOMA
somatic ras/raf mutations: KRAS p.G12D
age at diagnosis: 23
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted from tissues (epithelium and stroma) microdissected mucinous ovarian tumours stained with haematoxylin and eosin, and from lymphcoytes obtained from peripheral blood (for matched normal DNA) using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and Qubit.
Label BIOTIN
Label protocol As per manufacturer's recommendations (Affymetrix)
 
Hybridization protocol DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
Description HYBRIDISED TO SNP6
Data processing CHP files were generated using the Affymetrix Genotyping Console algorithm Birdseed v2. Total and allele-specific copy number analysis was performed using Partek Genomics Suite 6.5, using both paired copy number generation.
 
Submission date Jun 17, 2014
Last update date Mar 01, 2015
Contact name Sally Hunter
E-mail(s) Sally.Hunter@petermac.org
Organization name Peter MacCallum Cancer Centre
Department Research
Lab Cancer Genetics
Street address 7 St Andrew's Pl
City Melbourne
State/province VIC
ZIP/Postal code 3002
Country Australia
 
Platform ID GPL6801
Series (1)
GSE58579 High-resolution genome-wide copy number analysis of low grade serous ovarian tumours

Data table header descriptions
ID_REF
VALUE SNP6 normalized copy number data

Data table
ID_REF VALUE
AFFX-SNP_10000979 1.70142
AFFX-SNP_10009702 1.63479
AFFX-SNP_10015773 1.98083
AFFX-SNP_10021569 1.51108
AFFX-SNP_10026879 1.49187
AFFX-SNP_10029725 2.05894
AFFX-SNP_10034687 1.76899
AFFX-SNP_10036267 1.94428
AFFX-SNP_10037362 2.69203
AFFX-SNP_10037636 1.83769
AFFX-SNP_10039349 1.69494
AFFX-SNP_10039442 2.15661
AFFX-SNP_10039933 1.78607
AFFX-SNP_10046830 3.11042
AFFX-SNP_10047363 2.25847
AFFX-SNP_10047841 2.74199
AFFX-SNP_10049334 1.507
AFFX-SNP_10052664 1.74772
AFFX-SNP_10057887 2.20934
AFFX-SNP_10064911 2.43356

Total number of rows: 1855448

Table truncated, full table size 36332 Kbytes.




Supplementary file Size Download File type/resource
GSM1414423_IC462T.CEL.gz 27.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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