|
Status |
Public on Jul 31, 2014 |
Title |
genomic DNA from leukemic blast 5729917045_RO3CO1 |
Sample type |
genomic |
|
|
Source name |
leukemic blast (MNC)
|
Organism |
Homo sapiens |
Characteristics |
sample id: CNAML15 gender: Male age at diagnosis in years: 57 batch: batch1 cell type: leukemic blast
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA and RNA was extracted by TRIzol ( Invitrogen) or ALLPrep ( QIAGEN ) according to the standard instructions. Sample quanlity were tested by Genomic DNA ScreenTape.
|
Label |
Cy5 and Cy35
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
leukemic blast
|
Data processing |
The raw data was processed by GenomeStudio (QIAGEN) by standard protocol.
|
|
|
Submission date |
Jun 13, 2014 |
Last update date |
Jul 31, 2014 |
Contact name |
Ying Qu |
Organization name |
Karolinska Institute
|
Department |
Department of Medicine, Huddinge
|
Lab |
Center of Hematology and Regenerative Medicine (HERM)
|
Street address |
Hälsovagen 7
|
City |
Stockholm |
State/province |
Stockholm |
ZIP/Postal code |
14157 |
Country |
Sweden |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE58477 |
Differential methylation in CN-AML preferentially targets non-CGI regions and is dictated by DNMT3A mutational status and associated with predominant hypomethylation of HOX genes |
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