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Sample GSM1407112 Query DataSets for GSM1407112
Status Public on Nov 19, 2015
Title SARS_VEROlowMOI_24hr_3
Sample type SRA
 
Source name Vero E6 cells
Organism Chlorocebus sabaeus
Characteristics cell line: Vero E6
Treatment protocol RNA was isolated at 24 and 48 hours post infection at an MOI of 0.1 and 3 with each virus.
Growth protocol Vero E6 cells were grown in MEM with 10% Fetal Bovine Serum
Extracted molecule total RNA
Extraction protocol RNA was extracted using Trizol from cells infected in culture.
Poly-A RNA was isolated and indexed libraries were prepared for sequencing using standard Illumina protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing NOT PROVIDED
Genome_build: NOT PROVIDED
Supplementary_files_format_and_content: NOT PROVIDED
 
Submission date Jun 09, 2014
Last update date May 15, 2019
Contact name David A Rasko
E-mail(s) drasko@som.umaryland.edu
Phone 410 706 6774
Organization name University of Maryland School of Medicine
Department Istitute for Genome Sciences/Microbiology and Immunology
Street address 801 W. Baltimore Street, Room 619
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
 
Platform ID GPL18769
Series (2)
GSE56189 Transcriptomic Analysis Of The Novel Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
GSE56193 Transcriptomic analysis of the Novel Middle East Respiratory Syndrome Coronavirus (African green monkey, Vero E6 cells)
Relations
SRA SRX492435
SRA SRX492436
SRA SRX492437
BioSample SAMN02689487

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data not provided for this record

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