|
Status |
Public on Nov 19, 2015 |
Title |
Vero-3_20ulEMC_48hrs_RNA2 |
Sample type |
SRA |
|
|
Source name |
Vero E6 cells
|
Organism |
Chlorocebus sabaeus |
Characteristics |
cell line: Vero E6
|
Treatment protocol |
RNA was isolated at 24 and 48 hours post infection at an MOI of 0.1 and 3 with each virus.
|
Growth protocol |
Vero E6 cells were grown in MEM with 10% Fetal Bovine Serum
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Trizol from cells infected in culture. Poly-A RNA was isolated and indexed libraries were prepared for sequencing using standard Illumina protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
NOT PROVIDED Genome_build: NOT PROVIDED Supplementary_files_format_and_content: NOT PROVIDED
|
|
|
Submission date |
Jun 09, 2014 |
Last update date |
May 15, 2019 |
Contact name |
David A Rasko |
E-mail(s) |
drasko@som.umaryland.edu
|
Phone |
410 706 6774
|
Organization name |
University of Maryland School of Medicine
|
Department |
Istitute for Genome Sciences/Microbiology and Immunology
|
Street address |
801 W. Baltimore Street, Room 619
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21201 |
Country |
USA |
|
|
Platform ID |
GPL18770 |
Series (2) |
GSE56189 |
Transcriptomic Analysis Of The Novel Middle East Respiratory Syndrome Coronavirus (MERS-CoV) |
GSE56193 |
Transcriptomic analysis of the Novel Middle East Respiratory Syndrome Coronavirus (African green monkey, Vero E6 cells) |
|
Relations |
SRA |
SRX492687 |
SRA |
SRX492688 |
BioSample |
SAMN02689565 |