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Sample GSM1401391 Query DataSets for GSM1401391
Status Public on Dec 31, 2014
Title WholeEye_myopia_rep5
Sample type RNA
 
Source name whole eye, myopic, replicate5
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: whole eye
phenotype: myopic
age: 34 days
Treatment protocol C57BL/6J mice were purchased from the Jackson Laboratory. Form-deprivation was induced in fifteen postnatal day 24 (P24) mice by applying a unilateral frosted hemispherical plastic diffuser over the right eyes for ten days. The contralateral untreated left eyes were used as control. At the end of form-deprivation induction (P34) both myopic and control eyes were isolated and retina and sclera were dissected out in RNAlatter.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the whole eye, retina and sclera using mirVanaTM miRNA kit (Applied Biosystems, Austin, Texas) according to the manufacturer’s instructions. Total RNA concentration was measured using NanoDrop spectrophotometry. The quality of the total RNA was assessed using the Agilent RNA 600 Nano kit (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions.
Label Cy3
Label protocol Agilent miRNA Microarray System with miRNA Complete Labeling and Hyb Kit was used for the labeling protocol.
 
Hybridization protocol Agilent miRNA Microarray System with miRNA Complete Labeling and Hyb Kit for the hybridization protocol. Ozone covers were used.
Scan protocol Agilent B Scanner, Scan area (61 x 21.6mm), Scan resolution 5um, extended Dynamic range selected, Dye channel green, Green PMT XDR Hi 100%, XDR Lo 5%
Description miRNA expression after 10 days without FDM induction
MmiRNA-M-005
Data processing Feature Extraction version 11, protocol miRNA_1100_Jul11
 
Submission date May 30, 2014
Last update date Dec 31, 2014
Contact name Terri Young
Organization name Duke University
Department Ophthalmology
Street address LaSale St.
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL15880
Series (1)
GSE58124 Evaluation of MicroRNA Expression Profiles for Form-Deprivation Myopia in Mouse

Data table header descriptions
ID_REF
VALUE Partek Genomics Suite 6.6 (Partek Inc., St. Louis, MO) log2 normalized the Agilent miRNA data files.

Data table
ID_REF VALUE
A_54_P00001283 2.23491
A_54_P00001284 3.00979
A_54_P00002291 7.62068
A_54_P00002292 8.76303
A_54_P00002760 2.46066
A_54_P00002761 2.66931
A_54_P00002768 3.87842
A_54_P00002769 3.97695
A_54_P00004422 3.15497
A_54_P00004423 3.40154
A_54_P00004424 2.37178
A_54_P00004425 2.76035
A_54_P00004426 2.79032
A_54_P00004427 3.10119
A_54_P00004428 2.99019
A_54_P00004430 5.82897
A_54_P00004431 6.35133
A_54_P00004432 3.88223
A_54_P00004433 3.28816
A_54_P00004434 2.60249

Total number of rows: 1937

Table truncated, full table size 36 Kbytes.




Supplementary file Size Download File type/resource
GSM1401391_3226_11661_MmiRNA_M5_252915210226_S01_miRNA_1100_Jul11_1_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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