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Sample GSM1399443 Query DataSets for GSM1399443
Status Public on May 28, 2014
Title MoDC Young_4
Sample type RNA
Source name MoDC from age_20 donor
Organism Homo sapiens
Characteristics individual: young donor
age: 20
cell type: monocyte derived dendritic cells (MoDC)
Treatment protocol Purified CD14+ monocytes were cultured in RPMI 1640 10% fetal bovine serum. Supplemented with human rGM-CSF,50ng/ml and 10 ng/ml human rIL-4.
Growth protocol To induce differentiation of DCs, purified CD14+ monocytes were cultured in RPMI 1640 10% fetal bovine serum. Supplemented with human rGM-CSF,50ng/ml and 10 ng/ml human rIL-4 in 37C incubator with 5% CO2 for 6 days.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from MoDCs using the TRI Reagent kit (Molecular Research Center Inc., Cincinnati, OH, USA), following the manufacturer’s protocol.
Label Biotin
Label protocol The single-stranded cRNA sample is fragmented (WT Terminal Labeling Kit, Affymetrix, Inc, Santa Clara, CA) to an average strand length of 70 bases (range 40-70bp) following prescribed protocols (Affymetrix GeneChip WT Sense Target Labeling Assay Manual). The fragmented single-stranded DNA is subsequently labeled with recombinant terminal deoxynucleotidyl transferase (TdT) and the Affymetrix proprietary DNA Labeling Reagent that is covalently linked to biotin.
Hybridization protocol 2 ug of fragmented target single-stranded cRNA was hybridized at 45c with rotation for 17 hours (Affymetrix GeneChip® Hybridization Oven 640) to probe sets present on an Affymetrix array. The GeneChip® arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fludics Station 450 (Fluidics protocol FS450_007).
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G and Command Console Software v. produce cell intensity files (CEL). CEL were analyzed with Microarray Suite 5.0
Description Gene expression data in MoDC from young donor
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date May 28, 2014
Last update date May 28, 2014
Contact name Sudhir Gupta
Organization name University of California, Irvine
Department Medicine
Lab Immunology
Street address Med Sci Buld I, C240
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
Platform ID GPL571
Series (1)
GSE58015 Gene Expression Data in Human Monocyte-derived Dendritic Cell (MoDC) from Young and Aged Donors

Data table header descriptions
VALUE MAS5.0 signal

Data table
1007_s_at 101.3157
1053_at 323.0435
117_at 24.33888
121_at 511.5239
1255_g_at 1396.628
1294_at 602.8234
1316_at 27.73725
1320_at 3.763988
1405_i_at 2659.235
1431_at 16.01046
1438_at 32.18727
1487_at 633.2397
1494_f_at 37.54689
1598_g_at 1612.648
160020_at 263.2983
1729_at 1252.141
1773_at 111.1426
177_at 43.47168
179_at 218.2535
1861_at 247.7601

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.

Supplementary file Size Download File type/resource
GSM1399443_MoDC_Young_4.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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