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Status
Public on Oct 02, 2014
Title
patient BH003, low responder, female, day4, hour 4, recovery
Sample type
RNA
Source name
blood sample from patient BH003, low responder, female, day4, hour 4, recovery
Organism
Homo sapiens
Characteristics
patient: BH003 responder: low Sex: female day: 4 hour: 4 biological group: recovery
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH003, lowresponder, female, day4, hour 4, recovery
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
6.040162
100121620_TGI_at
7.59792
100121621_TGI_at
5.765958
100121622_TGI_at
8.074569
100121623_TGI_at
9.135024
100121624_TGI_at
7.66133
100121625_TGI_at
8.537509
100121626_TGI_at
3.9088
100121627_TGI_at
5.365308
100121628_TGI_at
4.092795
100121629_TGI_at
9.867893
100121630_TGI_at
6.029595
100121631_TGI_at
4.207041
100121632_TGI_at
8.146574
100121633_TGI_at
8.80291
100121634_TGI_at
4.383385
100121635_TGI_at
8.499425
100121636_TGI_at
5.567716
100121637_TGI_at
6.197554
100121638_TGI_at
5.300349
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371823__52070900797045031010407111221894_1_1.CEL.gz
5.0 Mb
(ftp) (http)
CEL
Processed data included within Sample table