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Status
Public on Oct 02, 2014
Title
patient BH003, low responder, female, day2, hour 8, baseline
Sample type
RNA
Source name
blood sample from patient BH003, low responder, female, day2, hour 8, baseline
Organism
Homo sapiens Characteristics
patient: BH003
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH003, lowresponder, female, day2, hour 8, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379 Series (1)
GSE56931 Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions ID_REF VALUE RMA expression measures from R/affy package
Data table ID_REF VALUE 100121619_TGI_at
5.785972
100121620_TGI_at
7.559859
100121621_TGI_at
6.877674
100121622_TGI_at
7.88368
100121623_TGI_at
8.72062
100121624_TGI_at
7.793574
100121625_TGI_at
8.308273
100121626_TGI_at
3.785553
100121627_TGI_at
5.288307
100121628_TGI_at
4.017179
100121629_TGI_at
9.828031
100121630_TGI_at
6.505846
100121631_TGI_at
4.508329
100121632_TGI_at
8.118876
100121633_TGI_at
8.944314
100121634_TGI_at
3.471386
100121635_TGI_at
8.594567
100121636_TGI_at
5.54888
100121637_TGI_at
6.768717
100121638_TGI_at
5.602674
52378 1335 Kbytes .Supplementary file Size Download File type/resource GSM1371821__52070900797045031010407111221890_1_1.CEL.gz
4.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
