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Status
Public on Oct 02, 2014
Title
patient BH010, low responder, female, day3, hour 4, sleepdep
Sample type
RNA
Source name
blood sample from patient BH010, low responder, female, day3, hour 4, sleepdep
Organism
Homo sapiens
Characteristics
patient: BH010 responder: low Sex: female day: 3 hour: 4 biological group: sleep deprivation
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH010, lowresponder, female, day3, hour 4, sleepdep
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
6.113584
100121620_TGI_at
7.092299
100121621_TGI_at
6.100698
100121622_TGI_at
8.27034
100121623_TGI_at
8.698315
100121624_TGI_at
7.903838
100121625_TGI_at
8.369765
100121626_TGI_at
4.176961
100121627_TGI_at
5.320503
100121628_TGI_at
3.582551
100121629_TGI_at
9.886941
100121630_TGI_at
5.949552
100121631_TGI_at
4.338263
100121632_TGI_at
7.528148
100121633_TGI_at
9.054267
100121634_TGI_at
3.456543
100121635_TGI_at
8.683652
100121636_TGI_at
5.278748
100121637_TGI_at
5.647557
100121638_TGI_at
5.312621
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371805__52070900797045031010407111221873_1_1.CEL.gz
4.9 Mb
(ftp) (http)
CEL
Processed data included within Sample table