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Status
Public on Oct 02, 2014
Title
patient BH015, high responder, male, day3, hour 4, sleepdep
Sample type
RNA
Source name
blood sample from patient BH015, high responder, male, day3, hour 4, sleepdep
Organism
Homo sapiens
Characteristics
patient: BH015 responder: high Sex: male day: 3 hour: 4 biological group: sleep deprivation
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH015, highresponder, male, day3, hour 4, sleepdep
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
6.411598
100121620_TGI_at
7.906489
100121621_TGI_at
5.160861
100121622_TGI_at
6.428145
100121623_TGI_at
8.691381
100121624_TGI_at
7.125456
100121625_TGI_at
7.751443
100121626_TGI_at
4.534874
100121627_TGI_at
5.765146
100121628_TGI_at
4.360798
100121629_TGI_at
9.282512
100121630_TGI_at
5.811362
100121631_TGI_at
3.917193
100121632_TGI_at
8.047679
100121633_TGI_at
9.348818
100121634_TGI_at
4.479301
100121635_TGI_at
8.504167
100121636_TGI_at
6.01597
100121637_TGI_at
5.99482
100121638_TGI_at
5.342501
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371709__52070900797042031010407111221700_1_1.CEL.gz
4.8 Mb
(ftp) (http)
CEL
Processed data included within Sample table