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Status
Public on Oct 02, 2014
Title
patient BH020, low responder, male, day2, hour 8, baseline
Sample type
RNA
Source name
blood sample from patient BH020, low responder, male, day2, hour 8, baseline
Organism
Homo sapiens
Characteristics
patient: BH020 responder: low Sex: male day: 2 hour: 8 biological group: baseline
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH020, lowresponder, male, day2, hour 8, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
6.157628
100121620_TGI_at
7.30153
100121621_TGI_at
6.239111
100121622_TGI_at
7.414431
100121623_TGI_at
8.585262
100121624_TGI_at
7.797226
100121625_TGI_at
8.134964
100121626_TGI_at
4.466133
100121627_TGI_at
5.409365
100121628_TGI_at
4.390777
100121629_TGI_at
9.63815
100121630_TGI_at
6.073447
100121631_TGI_at
4.762311
100121632_TGI_at
7.714774
100121633_TGI_at
9.363716
100121634_TGI_at
3.798758
100121635_TGI_at
8.68152
100121636_TGI_at
5.724113
100121637_TGI_at
5.918848
100121638_TGI_at
5.369217
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371659__52070900797040031010407111021382_1_1.CEL.gz
5.0 Mb
(ftp) (http)
CEL
Processed data included within Sample table