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Status
Public on Oct 02, 2014
Title
patient BH020, low responder, male, day3, hour 4, sleepdep
Sample type
RNA
Source name
blood sample from patient BH020, low responder, male, day3, hour 4, sleepdep
Organism
Homo sapiens Characteristics
patient: BH020
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH020, lowresponder, male, day3, hour 4, sleepdep
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379 Series (1)
GSE56931 Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions ID_REF VALUE RMA expression measures from R/affy package
Data table ID_REF VALUE 100121619_TGI_at
6.762692
100121620_TGI_at
7.380067
100121621_TGI_at
6.290486
100121622_TGI_at
7.650937
100121623_TGI_at
8.548472
100121624_TGI_at
8.085227
100121625_TGI_at
8.2726
100121626_TGI_at
3.900859
100121627_TGI_at
5.515923
100121628_TGI_at
4.170093
100121629_TGI_at
9.676789
100121630_TGI_at
5.932678
100121631_TGI_at
4.429736
100121632_TGI_at
7.454194
100121633_TGI_at
9.492092
100121634_TGI_at
3.851879
100121635_TGI_at
8.933291
100121636_TGI_at
5.340966
100121637_TGI_at
6.348843
100121638_TGI_at
5.241522
52378 1335 Kbytes .Supplementary file Size Download File type/resource GSM1371641__52070900797038031010407111021279_1_1.CEL.gz
5.0 Mb
(ftp) (http) CEL
Processed data included within Sample table
