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Status
Public on Oct 02, 2014
Title
patient BH020, low responder, male, day2, hour 4, baseline
Sample type
RNA
Source name
blood sample from patient BH020, low responder, male, day2, hour 4, baseline
Organism
Homo sapiens Characteristics
patient: BH020
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH020, lowresponder, male, day2, hour 4, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379 Series (1)
GSE56931 Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions ID_REF VALUE RMA expression measures from R/affy package
Data table ID_REF VALUE 100121619_TGI_at
6.622897
100121620_TGI_at
7.448783
100121621_TGI_at
5.659432
100121622_TGI_at
7.484793
100121623_TGI_at
8.525977
100121624_TGI_at
8.136402
100121625_TGI_at
8.04069
100121626_TGI_at
3.729902
100121627_TGI_at
5.763024
100121628_TGI_at
4.034104
100121629_TGI_at
9.606813
100121630_TGI_at
6.007328
100121631_TGI_at
4.470549
100121632_TGI_at
7.759103
100121633_TGI_at
9.278028
100121634_TGI_at
3.691329
100121635_TGI_at
8.792233
100121636_TGI_at
5.461133
100121637_TGI_at
6.220146
100121638_TGI_at
5.390952
52378 1335 Kbytes .Supplementary file Size Download File type/resource GSM1371639__52070900797038031010407111021262_1_1.CEL.gz
5.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
