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Status
Public on Oct 02, 2014
Title
patient BH009, low responder, female, day3, hour 0, sleepdep
Sample type
RNA
Source name
blood sample from patient BH009, low responder, female, day3, hour 0, sleepdep
Organism
Homo sapiens Characteristics
patient: BH009
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH009, lowresponder, female, day3, hour 0, sleepdep
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379 Series (1)
GSE56931 Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions ID_REF VALUE RMA expression measures from R/affy package
Data table ID_REF VALUE 100121619_TGI_at
6.360663
100121620_TGI_at
6.883579
100121621_TGI_at
6.820699
100121622_TGI_at
7.95515
100121623_TGI_at
8.642307
100121624_TGI_at
7.864631
100121625_TGI_at
8.113086
100121626_TGI_at
3.963059
100121627_TGI_at
5.466459
100121628_TGI_at
4.714653
100121629_TGI_at
9.627892
100121630_TGI_at
6.14195
100121631_TGI_at
4.425194
100121632_TGI_at
7.71995
100121633_TGI_at
9.255025
100121634_TGI_at
3.481563
100121635_TGI_at
8.760217
100121636_TGI_at
5.289196
100121637_TGI_at
5.735719
100121638_TGI_at
5.745714
52378 1335 Kbytes .Supplementary file Size Download File type/resource GSM1371625__52070900797037031010407111021223_1_1.CEL.gz
5.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
