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Status
Public on Oct 02, 2014
Title
patient BH014, low responder, male, day2, hour 8, baseline
Sample type
RNA
Source name
blood sample from patient BH014, low responder, male, day2, hour 8, baseline
Organism
Homo sapiens
Characteristics
patient: BH014 responder: low Sex: male day: 2 hour: 8 biological group: baseline
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH014, lowresponder, male, day2, hour 8, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379
Series (1)
GSE56931
Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions
ID_REF
VALUE
RMA expression measures from R/affy package
Data table
ID_REF
VALUE
100121619_TGI_at
6.049108
100121620_TGI_at
8.013067
100121621_TGI_at
6.125447
100121622_TGI_at
7.186484
100121623_TGI_at
8.825365
100121624_TGI_at
7.478936
100121625_TGI_at
8.036021
100121626_TGI_at
4.131795
100121627_TGI_at
5.371718
100121628_TGI_at
4.120411
100121629_TGI_at
9.749067
100121630_TGI_at
5.320485
100121631_TGI_at
4.253651
100121632_TGI_at
7.92231
100121633_TGI_at
8.948099
100121634_TGI_at
5.065999
100121635_TGI_at
8.496767
100121636_TGI_at
6.09814
100121637_TGI_at
5.483172
100121638_TGI_at
5.031228
Total number of rows: 52378 Table truncated, full table size 1335 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1371622__52070900797037031010407111021215_1_1.CEL.gz
4.9 Mb
(ftp) (http)
CEL
Processed data included within Sample table