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Status
Public on Oct 02, 2014
Title
patient BH014, low responder, male, day4, hour 4, recovery
Sample type
RNA
Source name
blood sample from patient BH014, low responder, male, day4, hour 4, recovery
Organism
Homo sapiens Characteristics
patient: BH014
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH014, lowresponder, male, day4, hour 4, recovery
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379 Series (1)
GSE56931 Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions ID_REF VALUE RMA expression measures from R/affy package
Data table ID_REF VALUE 100121619_TGI_at
6.214626
100121620_TGI_at
7.709697
100121621_TGI_at
5.845001
100121622_TGI_at
7.60307
100121623_TGI_at
8.829464
100121624_TGI_at
7.475879
100121625_TGI_at
8.016948
100121626_TGI_at
4.120841
100121627_TGI_at
5.587194
100121628_TGI_at
4.820803
100121629_TGI_at
9.883925
100121630_TGI_at
6.088625
100121631_TGI_at
4.265674
100121632_TGI_at
7.893477
100121633_TGI_at
8.95397
100121634_TGI_at
3.624363
100121635_TGI_at
8.495258
100121636_TGI_at
5.628506
100121637_TGI_at
6.118199
100121638_TGI_at
5.582599
52378 1335 Kbytes .Supplementary file Size Download File type/resource GSM1371616__52070900797037031010407111021204_1_1.CEL.gz
5.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
