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Status
Public on Oct 02, 2014
Title
patient BH014, low responder, male, day2, hour 0, baseline
Sample type
RNA
Source name
blood sample from patient BH014, low responder, male, day2, hour 0, baseline
Organism
Homo sapiens Characteristics
patient: BH014
Extracted molecule
total RNA
Extraction protocol
PAXgene protocol was used to extract total RNA
Label
biotin
Label protocol
RT/IVT protocol and reagents provided by Affymetrix
Hybridization protocol
Following fragmentation, 10 ug of cDNA was hybridized for 18 hr at 45C. GeneChips were washed and stained in the Affymetrix
Scan protocol
Affymetrix GeneChip Scanner 3000
Description
blood sample from patient BH014, lowresponder, male, day2, hour 0, baseline
Data processing
Data were processed using R/affy software. In brief, the RMA method adjusts the background of perfect match (PM) probes, applies a quantile normalization of the corrected PM values, and calculates final expression measures with the Tukey median polish algorithm.
Submission date
Apr 21, 2014
Last update date
Oct 03, 2014
Contact name
Keith Shockley
Organization name
NIEHS
Street address
111 TW Alexander Drive
City
RTP
State/province
NC
ZIP/Postal code
27709
Country
USA
Platform ID
GPL10379 Series (1)
GSE56931 Blood gene expression reveals reduced circadian rhythmicity in individuals resistant to sleep deprivation
Data table header descriptions ID_REF VALUE RMA expression measures from R/affy package
Data table ID_REF VALUE 100121619_TGI_at
6.130706
100121620_TGI_at
7.81079
100121621_TGI_at
5.521081
100121622_TGI_at
7.580018
100121623_TGI_at
8.990839
100121624_TGI_at
7.060132
100121625_TGI_at
8.143784
100121626_TGI_at
3.868294
100121627_TGI_at
5.382284
100121628_TGI_at
4.442377
100121629_TGI_at
9.982682
100121630_TGI_at
5.353407
100121631_TGI_at
4.429749
100121632_TGI_at
7.674844
100121633_TGI_at
9.070026
100121634_TGI_at
4.47327
100121635_TGI_at
8.228633
100121636_TGI_at
5.445032
100121637_TGI_at
6.420475
100121638_TGI_at
5.269866
52378 1335 Kbytes .Supplementary file Size Download File type/resource GSM1371614__52070900797037031010407111021202_1_1.CEL.gz
5.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
