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Sample GSM1369393 Query DataSets for GSM1369393
Status Public on Nov 17, 2014
Title Input DNA
Sample type SRA
Source name HEK293 Cells
Organism Homo sapiens
Characteristics antibody 1: none
antibody 2: none
cell line: HEK293
cell type: embryonic kidney
Growth protocol HEK293 were grown under standard tissue culture conditions
Extracted molecule genomic DNA
Extraction protocol Lysates were clarified from sonicated HEK293 nuclei and histone-DNA complexes were isolated with anti-DDX21 antibody.
Libraries were prepared according to NEBNext kit.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2500
Data processing ChIP-seq reads were aligned to the hg19 genome assembly using DNANexus
peaks were called using QuEST with the following setting: ChIP estimate threshold (20),ChIP extension fold enrichment (3), and a ChIP-to-background fold enrichment (3)
Genome_build: hg19
Submission date Apr 15, 2014
Last update date May 15, 2019
Contact name Ryan Alexander Flynn
Organization name Stanford University
Street address 380 Roth Way, Room 265
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL16791
Series (1)
GSE56802 RNA helicase DDX21 coordinates transcription and noncoding RNA processing of the ribosomal pathway
BioSample SAMN02727890
SRA SRX952173

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data not provided for this record
Processed data are available on Series record

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