|
| Status |
Public on Jun 11, 2014 |
| Title |
X. tropicalis brain small RNAs |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Xenopus tropicalis |
| Characteristics |
Sex: Male
individual: M2
tissue type: brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 20-30 mg of tissue, using the QIAgen miRNeasy mini kit according to the manufacturer's instructions. The RNA quality was checked using a Fragment Analyzer.
2ug of total RNA were used to prepare small RNA libraries: after a purification of the small RNA on a 15% TBU acrylamide gel, the libraries were generated using the Illumina TruSeq(R) Small RNA Sample Prep Kit and the quality assessed using a Fragment Analyzer.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
small RNA
|
| Data processing |
There are no processed data files associated with this submission. This is because the data were only used to test presence/absence of 15 transcripts that had been identified based on published datasets. We did not run any genome-wide expression analysis or similar.
|
| |
|
| Submission date |
Apr 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Maria Warnefors |
| Organization name |
University of Heidelberg
|
| Street address |
Center for Molecular Biology of Heidelberg University (ZMBH)
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18470 |
| Series (1) |
| GSE56680 |
MicroRNA editing in Xenopus tropicalis |
|
| Relations |
| BioSample |
SAMN02725059 |
| SRA |
SRX514968 |
