|
| Status |
Public on Apr 07, 2015 |
| Title |
Ovarian cancer cell TGF-beta treated 120+48 hours (rep2) |
| Sample type |
genomic |
| |
|
| Source name |
cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKOV3
cell type: ovarian cancer
treatment: TGF-beta 120+48H
|
| Treatment protocol |
Cells were treated with TGF-beta at the dose of 5 ng/ml.
|
| Growth protocol |
Cells were maintained in medium DMGM.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from cells using Qiagen QiaAMP kit according to standard instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Infinium HD Methylation Assay Protocol
|
| |
|
| Hybridization protocol |
DNA was amplified, fragmented, and hybridized to Illumina Infinium HumanMethylation450 BeadChip using standard Infinium HD Methylation Assay protocol.
|
| Scan protocol |
Arrays were imaged with HiScan Reader using recommended Illumina scanner settings.
|
| Description |
Ovarian cancer cells stimulated with TGF-beta for 120 hours then 48 hours off.
|
| Data processing |
GenomeStudio v2011.2 with Methylation module v1.8.5
Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
Apr 08, 2014 |
| Last update date |
Apr 07, 2015 |
| Contact name |
Horacio Cardenas |
| E-mail(s) |
horacard@iu.edu
|
| Phone |
317-274-7305
|
| Organization name |
Indiana University
|
| Street address |
980 West Walnut Street
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE56621 |
TGF-beta stimulation in ovarian cancer cells |
|
