|
Status |
Public on Feb 23, 2015 |
Title |
0879C |
Sample type |
RNA |
|
|
Source name |
B-cell acute lymphoblastic leukemia with other cytogenetics
|
Organism |
Homo sapiens |
Characteristics |
tissue: bone marrow / peripheral blood subjects: children with B-ALL (California Childhood Cancer Study) age: 3.6 gender: M cytogenetics: others
|
Treatment protocol |
Bone marrow was isolated from B-ALL patients
|
Growth protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer's instructions
|
Label |
biotin
|
Label protocol |
1 μg of high-quality total RNA was amplified and labeled (NuGen Ovation)
|
|
|
Hybridization protocol |
Following fragmentation, cRNA were hybridized onto GeneChip Human Gene 1.0 ST Array (Affymetrix) according to Affymetrix's instructions.
|
Scan protocol |
GeneChips were scanned using the Affymetrix genechip scanner
|
Description |
Gene expression data of B-cell acute lymphoblastic leukemia cells B-ALL, others
|
Data processing |
Data was processed and normalized using the Expression Console software (Affymetrix) with the standard Robust Multi-array Average (RMA) algorithm
|
|
|
Submission date |
Apr 08, 2014 |
Last update date |
Feb 23, 2015 |
Contact name |
Seung-Tae Lee |
E-mail(s) |
essere.lee@gmail.com
|
Phone |
+82-10-9933-5465
|
Organization name |
UCSF
|
Street address |
1450 3rd Street
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (2) |
GSE56599 |
Expression data of pediatric B-cell acute leukemias |
GSE56602 |
Two-Track Epigenetic Remodeling and Backtracking to Embryonic Stem Cell Bivalency in B-cell Acute Lymphoblastic Leukemias |
|