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Sample GSM1365286 Query DataSets for GSM1365286
Status Public on Feb 23, 2015
Title 1539C
Sample type RNA
 
Source name B-cell acute lymphoblastic leukemia with ETV6/AML18 translocation
Organism Homo sapiens
Characteristics tissue: bone marrow / peripheral blood
subjects: children with B-ALL (California Childhood Cancer Study)
age: 3.6
gender: M
cytogenetics: ETV6/AML1
Treatment protocol Bone marrow was isolated from B-ALL patients
Growth protocol none
Extracted molecule total RNA
Extraction protocol AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer's instructions
Label biotin
Label protocol 1 μg of high-quality total RNA was amplified and labeled (NuGen Ovation)
 
Hybridization protocol Following fragmentation, cRNA were hybridized onto GeneChip Human Gene 1.0 ST Array (Affymetrix) according to Affymetrix's instructions.
Scan protocol GeneChips were scanned using the Affymetrix genechip scanner
Description Gene expression data of B-cell acute lymphoblastic leukemia cells
B-ALL, ETV6/AML1
Data processing Data was processed and normalized using the Expression Console software (Affymetrix) with the standard Robust Multi-array Average (RMA) algorithm
 
Submission date Apr 08, 2014
Last update date Feb 23, 2015
Contact name Seung-Tae Lee
E-mail(s) essere.lee@gmail.com
Phone +82-10-9933-5465
Organization name UCSF
Street address 1450 3rd Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL6244
Series (2)
GSE56599 Expression data of pediatric B-cell acute leukemias
GSE56602 Two-Track Epigenetic Remodeling and Backtracking to Embryonic Stem Cell Bivalency in B-cell Acute Lymphoblastic Leukemias

Data table header descriptions
ID_REF
VALUE RMA-normalized Signal intensity

Data table
ID_REF VALUE
7896736 7.037455
7896738 4.15895
7896740 3.771683
7896742 7.459273
7896744 5.391629
7896746 11.34431
7896748 11.38368
7896750 7.87806
7896752 11.53895
7896754 4.628826
7896756 6.25515
7896759 7.126984
7896761 7.139503
7896779 7.330032
7896798 7.185588
7896817 8.23863
7896822 6.806468
7896859 6.891304
7896861 4.707361
7896863 5.339526

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1365286_85.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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