|
| Status |
Public on Feb 23, 2015 |
| Title |
0727C |
| Sample type |
RNA |
| |
|
| Source name |
B-cell acute lymphoblastic leukemia with ETV6/AML10 translocation
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow / peripheral blood
subjects: children with B-ALL (California Childhood Cancer Study)
age: 3.9
gender: M
cytogenetics: ETV6/AML1
|
| Treatment protocol |
Bone marrow was isolated from B-ALL patients
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
1 μg of high-quality total RNA was amplified and labeled (NuGen Ovation)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized onto GeneChip Human Gene 1.0 ST Array (Affymetrix) according to Affymetrix's instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix genechip scanner
|
| Description |
Gene expression data of B-cell acute lymphoblastic leukemia cells
B-ALL, ETV6/AML1
|
| Data processing |
Data was processed and normalized using the Expression Console software (Affymetrix) with the standard Robust Multi-array Average (RMA) algorithm
|
| |
|
| Submission date |
Apr 08, 2014 |
| Last update date |
Feb 23, 2015 |
| Contact name |
Seung-Tae Lee |
| E-mail(s) |
essere.lee@gmail.com
|
| Phone |
+82-10-9933-5465
|
| Organization name |
UCSF
|
| Street address |
1450 3rd Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE56599 |
Expression data of pediatric B-cell acute leukemias |
| GSE56602 |
Two-Track Epigenetic Remodeling and Backtracking to Embryonic Stem Cell Bivalency in B-cell Acute Lymphoblastic Leukemias |
|
