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Sample GSM1365220 Query DataSets for GSM1365220
Status Public on Nov 30, 2014
Title S_471
Sample type RNA
 
Source name Vestibular Schwannoma
Organism Homo sapiens
Characteristics cell type: Vestibular Schwannoma
tissue type: Tumor
batch: 1
Treatment protocol Samples were frozen in liquid Nitrogen immediately after surgical resection, and then conserved at -80ºC until extraction
Extracted molecule total RNA
Extraction protocol RNA was isolated using RNeasy® Mini Kit (QIAGEN) following instructions. Rnase-Free Dnase Set (QIAGEN) was used to eliminate DNA contamitations.
Label biotin
Label protocol library preparation and amplification were performed following the distributor’s (Sigma-Aldrich) recommendations for whole transcriptome amplification (WTA2). Amplification was performed for 17 cycles, and amplified cDNA was purified and quantified on a NanoDrop ND-1000 spectrophotometer (Thermo-Fischer). Some 8 µg cDNA was subsequently fragmented by DNAseI and biotinylated by terminal transferase obtained from a GeneChip Mapping 10Kv2 Assay Kit (Affymetrix).
 
Hybridization protocol Hybridization, washing, staining and scanning of Affymetrix Human Gene ST 1.0 arrays were performed following the manufacturer's recommendations
Scan protocol GeneChip® Scanner 3000 7G
Description Previously analysed Vestibular Schwannoma 13
re-analysis of GSM973642
Data processing Scanned images (DAT files) were transformed into intensities (CEL files) by GCOS (Affymetrix).
HuGene-1_0-st-v1.r4.pgf
HuGene-1_0-st-v1.r4.mps
 
Submission date Apr 08, 2014
Last update date Nov 30, 2014
Contact name Miguel Torres-Martin
E-mail(s) migtorres.martin@gmail.com
Organization name Hospital Universitari Germans Trias i Pujol
Department Clinical Genomics Unit
Street address Carretera de Canyet, s/n
City Badalona
State/province Barcelona
ZIP/Postal code 08916
Country Spain
 
Platform ID GPL10739
Series (2)
GSE56597 Wide methylation analysis in vestibular schwannoma [Affymetrix exon level analysis]
GSE56598 Wide methylation analysis in vestibular schwannoma
Relations
Reanalysis of GSM973642

Data table header descriptions
ID_REF
VALUE Exon level analysis. RMA signal log2 converted from Expression Console Software 1.1.2800.29167. As samples were processes in two batches, ComBat method was used to remove batch effect.

Data table
ID_REF VALUE
7896739 4.212330898
7896741 4.196595876
7896743 7.550907834
7896745 5.324827145
7896747 8.107375558
7896749 7.75334223
7896751 3.790503154
7896753 6.228017148
7896755 6.848728296
7896757 7.294104551
7896758 7.114292627
7896760 8.074090659
7896762 11.28421026
7896763 6.248915856
7896764 5.876483179
7896765 4.964792294
7896766 8.545344611
7896767 6.116049069
7896768 10.35874115
7896769 6.611625642

Total number of rows: 257430

Table truncated, full table size 5000 Kbytes.




Supplementary file Size Download File type/resource
GSM1365220_1-471.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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