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Sample GSM1358435 Query DataSets for GSM1358435
Status Public on Mar 28, 2014
Title papain lung basophils #2
Sample type SRA
Source name Lung basophils
Organism Mus musculus
Characteristics strain: C57BL6/J
tissue: Lung
treatment: papain
Treatment protocol Mice were intranasally administrated with papain (50ug/mouse) for consective three days.
Growth protocol We sorted FceR1+,CD49b+,c-kit- cells as basophils,Lineage-,Thy1.2+,IL7Ra+,CD25+ cells as natural helper cells and SiglecF+,CD11c(+/-),Gr1lo,autofluorescence- cells as eosinophils from the lung by BD FACS Aria‚Ö°
Extracted molecule polyA RNA
Extraction protocol mRNA was isolated with TRIzol and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB Biolabs, Inc. Ipswich, MA,USA) .
10ng of isolated RNA were used for production of the RNA-Seq cDNA library with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB Biolabs, Inc.) according to the manufacturer's protocol that included cDNA synthesis and fragmentation, the addition of adaptors, size selection, amplification and quality control.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Data processing Read mapping,Bowtie0.12.7,Tophat v1.4.0
Estimate the transcript abundances,Cufflinks v1.3.0
Genome_build: NCBI Build 37(UCSC mm9)
Submission date Mar 27, 2014
Last update date May 15, 2019
Contact name Yasutaka Motomura
Organization name RIKEN
Department IMS
Lab Immune Cell System
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL18480
Series (1)
GSE56292 Expression profiling by high throughput sequencing
Reanalyzed by GSE80797
BioSample SAMN02709611
SRA SRX502140

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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