|
| Status |
Public on Mar 24, 2015 |
| Title |
total RNA from stimulated Tcell P7/R2 |
| Sample type |
RNA |
| |
|
| Source name |
Control-P7/R2 14 PD
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Stimulated T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with the Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification kit. Quality control was performed with the Agilent Bioanalyzer.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol. Illumina HT-12 V.3 arrays.
|
| Scan protocol |
Standard Illumina scanning protocol. Scanned in a BeadArray reader.
|
| Description |
Stimulated T cells
|
| Data processing |
The data were extracted with the HumanHT-12_V3_0_R2_11283641_A manifest and normalised using rank invariant normalization in the GenomeStudio Gene expression module.
|
| |
|
| Submission date |
Mar 20, 2014 |
| Last update date |
Mar 24, 2015 |
| Contact name |
Sofie Degerman |
| E-mail(s) |
sofie.degerman@umu.se
|
| Organization name |
Umeå University
|
| Department |
Medical Biosciensces
|
| Lab |
Pathology
|
| Street address |
NUS, Blg 6M, 2nd floor
|
| City |
Umeå |
| State/province |
Sweden |
| ZIP/Postal code |
90185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE56069 |
Immortalization of T-cells is accompanied by gradual changes in CpG methylation resulting in a profile resembling a subset of T-cell leukemias [expression] |
| GSE56070 |
Immortalization of T-cells is accompanied by gradual changes in CpG methylation resulting in a profile resembling a subset of T-cell leukemias |
|
