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Sample GSM135266 Query DataSets for GSM135266
Status Public on Aug 27, 2007
Title T-cell prolymphocytic leukemia, inv(14)(q11q32), case 3
Sample type RNA
 
Source name CD3-purified cells from T-PLL peripheral blood sample. >95% purity
Organism Homo sapiens
Characteristics Karyotype: inv(14)(q11q32)
Age: 72 years
Gender: male
Treatment protocol mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from a highly purified inv(14)-positive T-PLL blood sample
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
 
Submission date Sep 07, 2006
Last update date Aug 27, 2007
Contact name Ludger Klein-Hitpass
E-mail(s) ludger.klein-hitpass@uni-essen.de
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
 
Platform ID GPL96
Series (1)
GSE5788 Expression data from T-cell prolymphocytic leukemia (TPLL) and normal T cells

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 688 P 0.005565
AFFX-BioB-M_at 752 P 0.002023
AFFX-BioB-3_at 239.6 A 0.067678
AFFX-BioC-5_at 1943.1 P 0.000225
AFFX-BioC-3_at 1766.8 P 0.000044
AFFX-BioDn-5_at 2318.7 P 0.000127
AFFX-BioDn-3_at 8169.1 P 0.000258
AFFX-CreX-5_at 18913 P 0.000052
AFFX-CreX-3_at 27981.1 P 0.000044
AFFX-DapX-5_at 408.8 P 0.000195
AFFX-DapX-M_at 1114.4 P 0.001593
AFFX-DapX-3_at 1254.2 P 0.00039
AFFX-LysX-5_at 3027 P 0.000044
AFFX-LysX-M_at 4562.7 P 0.000052
AFFX-LysX-3_at 7198.5 P 0.000044
AFFX-PheX-5_at 17482.7 P 0.000044
AFFX-PheX-M_at 16565.9 P 0.000044
AFFX-PheX-3_at 22964.7 P 0.000044
AFFX-ThrX-5_at 3262 P 0.000044
AFFX-ThrX-M_at 3813 P 0.000044

Total number of rows: 22283

Table truncated, full table size 600 Kbytes.




Supplementary file Size Download File type/resource
GSM135266.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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