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Sample GSM1347989 Query DataSets for GSM1347989
Status Public on Jul 29, 2014
Title MSC donor9_TCP
Sample type genomic
 
Source name mesenchymal stromal cells (MSC)
Organism Homo sapiens
Characteristics cell type: MSC
donor: donor9
substrate: tissue culture plastic (TCP)
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was isolated using the Qiagen DNA Blood Midi Kit.
Label Cy5 and Cy3
Label protocol Standard Infinium HD Methylation Assay protocol
 
Hybridization protocol Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Infinium HD Methylation Assay protocol.
Scan protocol Standard Infinium HD Methylation Assay protocol
Data processing Initial analysis was performed by the GenomeStudio 2010.3 (Modul M Version 1.8.5). Data were normalized with internal controls according to Illumina┬┤s standard procedures. Methylation level at each locus was calculated with the GenomeStudio Methylation module as beta-value (ranging from 0 to 1). The number of beads per feature varies between chips and beta-values were calculated as average of at least three technical replica.
 
Submission date Mar 13, 2014
Last update date Jul 29, 2014
Contact name Wolfgang Wagner
E-mail(s) wwagner@ukaachen.de
Phone +49 241 8088611
Organization name RWTH Aachen University
Department Helmholtz Institute for Biomedical Engineering
Lab Stem Cell Biology and Cellular Engineering
Street address Pauwelsstrasse 20
City Aachen
ZIP/Postal code 52074
Country Germany
 
Platform ID GPL13534
Series (2)
GSE55888 Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells [DNAm profiling]
GSE55889 Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells

Data table header descriptions
ID_REF
VALUE normalized Average Beta
Detection Pval

Data table
ID_REF VALUE Detection Pval
cg00000029 0.1514962 0
cg00000108 0.8749132 0
cg00000109 0.5266207 0
cg00000165 0.2305253 0
cg00000236 0.7702248 0
cg00000289 0.3871973 0
cg00000292 0.4108009 0
cg00000321 0.1674056 0
cg00000363 0.1572418 0
cg00000622 0.01768225 0
cg00000658 0.7480476 0
cg00000714 0.08809923 0
cg00000721 0.8736387 0
cg00000734 0.0802526 0
cg00000769 0.05302223 0
cg00000807 0.7727294 0
cg00000884 0.7406334 0
cg00000905 0.1110704 0
cg00000924 0.4925009 0
cg00000948 0.8149458 0

Total number of rows: 485577

Table truncated, full table size 11010 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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