|
Status |
Public on Jul 29, 2014 |
Title |
MSC donor7_50 kPa |
Sample type |
genomic |
|
|
Source name |
mesenchymal stromal cells (MSC)
|
Organism |
Homo sapiens |
Characteristics |
cell type: MSC donor: donor7 substrate: 50 kPa PDMS
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using the Qiagen DNA Blood Midi Kit.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Infinium HD Methylation Assay protocol
|
|
|
Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450 Beadchip using standard Infinium HD Methylation Assay protocol.
|
Scan protocol |
Standard Infinium HD Methylation Assay protocol
|
Data processing |
Initial analysis was performed by the GenomeStudio 2010.3 (Modul M Version 1.8.5). Data were normalized with internal controls according to Illumina´s standard procedures. Methylation level at each locus was calculated with the GenomeStudio Methylation module as beta-value (ranging from 0 to 1). The number of beads per feature varies between chips and beta-values were calculated as average of at least three technical replica.
|
|
|
Submission date |
Mar 13, 2014 |
Last update date |
Jul 29, 2014 |
Contact name |
Wolfgang Wagner |
E-mail(s) |
wwagner@ukaachen.de
|
Phone |
+49 241 8088611
|
Organization name |
RWTH Aachen University
|
Department |
Helmholtz Institute for Biomedical Engineering
|
Lab |
Stem Cell Biology and Cellular Engineering
|
Street address |
Pauwelsstrasse 20
|
City |
Aachen |
ZIP/Postal code |
52074 |
Country |
Germany |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE55888 |
Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells [DNAm profiling] |
GSE55889 |
Matrix Elasticity Does Not Affect Replicative Senescence or DNA Methylation Patterns of Mesenchymal Stem Cells |
|