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Sample GSM1347956 Query DataSets for GSM1347956
Status Public on Sep 01, 2014
Title PyMT Tumor B
Sample type RNA
Source name mouse mammary tumor
Organism Mus musculus
Characteristics strain: FVB/N
genotype: PyMT
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen) and the RNEasy mini kit (Qiagen) with on-column DNAse digestion was used following Trizol extraction. 100ng total RNA per sample was used for array analysis.
Label biotin
Label protocol Fragmented ssDNA was labeled with the Affymetrix WT Terminal Labeling and Hybridization kit according to the instructions, using Affymetrix proprietary biotinylated labeling reagent.
Hybridization protocol Samples were hybridized using the Gene Atlas Microarray System (Affymetrix) hybridization chamber; 20 hours at 48 C
Scan protocol Samples were scanned and imaged using the Gene Atlas Microarray System Fludics and Imaging equipment (Affymetrix)
Data processing CEL files were processed in Partek Genomic Suite using RMA Quantile normalization; intensity values were scaled by log2 transformation
Submission date Mar 13, 2014
Last update date Sep 01, 2014
Contact name Elizabeth A Wellberg
Organization name University of Oklahoma HSC
Department Pathology
Street address 975 NE 10th St, BRC 309
City Oklahoma City
State/province Oklahoma
ZIP/Postal code 73104
Country USA
Platform ID GPL11533
Series (1)
GSE55886 Effect of Spot14 Loss on Gene Expression Profile of MMTV-PyMT Mouse Tumors

Data table header descriptions
VALUE RMA-normalized, log2 transformed intensity values

Data table
10338001 10.4446
10338002 4.94553
10338003 8.87917
10338004 8.11442
10338005 2.59155
10338006 2.783
10338007 2.98287
10338008 3.34158
10338009 6.31935
10338010 2.61131
10338011 4.46857
10338012 2.67023
10338013 2.48594
10338014 2.52355
10338015 2.47511
10338016 5.83787
10338017 11.2655
10338018 5.31629
10338019 4.11617
10338020 6.30023

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.

Supplementary file Size Download File type/resource 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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